SCMOS

“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
Utilizing selective plane illumination, Lightsheet microscopy is a fluorescence method that places a thin sheet of excitation light across only the focal plane being imaged. The sheet of light is swept in very precise synchronization to the rolling shutter of the camera. The scan height of the rolling shutter effectively becomes a slit, enabling less background signal and scattered light. By using this combination of illumination and camera readout, very fast acquisition of optically sectioned images is enabled. Additionally, there is significantly reduced photobleaching and phototoxicity to the sample as compared to many other methods.
Lightsheet microscopy is used for live cell imaging at scales that range from small groups of cells to whole organisms. The rapid increase in the use of this technique over the past several years has been remarkable.